Sensitive Mercury Speciation by Reversed-Phase Column High-Performance Liquid Chromatography with UV-Visible Detection After Solid-Phase Extraction Using 6-Mercaptopurine and Dithizone
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چکیده
منابع مشابه
Separation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملSeparation of Somatropin and Its Degradation Products by High-Performance Liquid Chromatography Using a Reversed-Phase Polymeric Column
The accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. In this study, a gradient RP-HPLC method is described for the separation of human growth hormone (hGH) variants as deamidated and oxidized forms. The methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1mL/min flow rate of a linear gradient of 0.1% v/...
متن کاملPolymer microchips integrating solid-phase extraction and high-performance liquid chromatography using reversed-phase polymethacrylate monoliths.
Polymer microfluidic chips employing in situ photopolymerized polymethacrylate monoliths for high-performance liquid chromatography separations of peptides is described. The integrated chip design employs a 15 cm long separation column containing a reversed-phase polymethacrylate monolith as a stationary phase, with its front end seamlessly coupled to a 5 mm long methacrylate monolith which fun...
متن کاملRapid speciation analysis of Cr(VI) and Cr(III) by reversed-phase high-performance liquid chromatography with UV detection.
A simple and rapid method is developed for the simultaneous determination of Cr(VI) and Cr(III) based on the formation of their different complexes with ammonium pyrrolidine-dithiocarbamate (APDC). Separation is performed using reversed-phase high-performance liquid chromatography coupled with UV detection. The conditions for complex formation and speciation are determined, such as solution pH,...
متن کاملseparation of somatropin and its degradation products by high-performance liquid chromatography using a reversed-phase polymeric column
the accurate prediction of protein stability is one of the most challenging goals in protein formulation and delivery. in this study, a gradient rp-hplc method is described for the separation of human growth hormone (hgh) variants as deamidated and oxidized forms. the methodology employed a polymeric poly (styrene-co-divinylbenzene) column and a 1ml/min flow rate of a linear gradient of 0.1% v/...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 2008
ISSN: 1060-3271,1944-7922
DOI: 10.1093/jaoac/91.6.1453